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Densities of liquids

Comments
 Supplement
Weight measurements are always much more precise than volume measurements. An electronic balance makes it not only more precise, but also more convenient.
    SubstanceDensity [g/ml]    Additional    
    information
    Acetic acid glacial1.049Tb=118oC
    Acetic acid 36%1.049 
    Acetone0.788Tb=56.5oC
    AcOK, 5.0M
    1.0M    		1.218
    1.046    		 
    AcONa, 3.0M
    2.5M    		1.121
    1.147    		 
    AcONH4, 10M1.073 
    Acrylamid:BIS, 40%1.044 
    Ammonium hydroxide, NH4OH, 28%0.89 
    Blocking reag., 10%1.038 
    Butanol (n)0.81Tb=117-118oC
    CaCl2, 1M1.086 
    Chloroform1.48Tb=61-62oC
    Denhardt sol., 50X1.004 
    DMFA0.945 
    DMSO1.100 
    EDTA, 0.5M1.096 
    Ethanol, anhydr
    96%
    70%    		0.79
    0.80
    0.868    		Tb=78.5oC
    FGRB, 50X1.104 
    Formaldehyde, 37%1.09 
    Formamid1.133 
    Glucose, 10%(w/v)
    2M    		1.040
    1.13    		 
    Glycerol1.25 
    GTGB, 20X1.094 
    HCl, 37% (12.07M)1.19 
    Isoamyl alcohol0.813Tb=132oC
    Isobutanol0.871Tb=108oC
    Isopropanol0.785Tb=82.5oC
    KCl, 0.25M1.044 
    KOH, 5.0M1.217 
    LB1.024 
    LiCl, 9M
    8M    		1.194
    1.172    		 
    Mercaptoethanol (ß)1.11414.192M,
    Mw 78.13
    MgCl2, 1M1.073 
    MgSO4, 1M1.112 
    MnCl2, 1M1.099 
    Nitric acid, HNO371%1.42 
    NaCl, 5M1.186 
    NaOH, 50%1.53 
    NaOH, 10M1.329 
    NaxHPO4, 1M,pH~71.104 
    Nonident P-401.064 
    Octane0.71Tb=125.6oC
    PEG 6000, 20%
    40%    		1.041
    1.075    		 
    PEG/NaCl, (20%/2.5M)1.145 
    PEG/TAE, (15%/1X)1.032 
    Phosphoric acid H3PO4, 85%1.70 
    Phenol1.054Tb=182oC,
    Tm=43oC
    Sulfuric acid H2SO4, 96%1.84 
    Sarcosyl, 10%(w/v)

    30%(w/v)

    		1.017
    1.027    		 
    SDS, 10%1.018 
    SSC, 20X
    30X    		1.163
    1.245    		 
    SSPE, 20X1.136 
    TAE, 50X1.088 
    TBE, 10X1.070 
    Tris.Cl, 1M, pH 7-8
    2M, pH7.9    		1.041
    1.10    		 
    TritonX-100, 10%1.016 
    TritonX-100, 100%1.06 
    Tween 20, 100%1.108 (1.101) 
    ZnCl2, 1M1.111 


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    Comments

  • We prefer to use a balance for preparing:

    • * agarose gels (weighing of agarose and buffer);
    * AA gels (weighing of all components);
    * 1x electrophoresis buffers from the stocks (weighing of water and stock solution);
    * salt solutions;
    * in the cases of viscous liquids, large volumes etc.

  • It is really convenient if there are two balances in the laboratory. One (that is used more often) is with the 0.01g gauge and the range to 1.5-3kg. It is used for preparation of large volumes of buffers, balancing of centrifuge tubes etc. The other balance has 0.0001g gauge and the range of 50-100g. It is used for preparation of small volumes of buffers (it is sufficient if the neighboring laboratory has such a scale).
  • If the density of the solution is known, it is possible to calculate the water volume (weight) that is necessary for its preparation. It is very convenient, because otherwise phrase "add water to the final volume…" forces you to use the graduated cylinder for the preparation of solution (calibration of all other laboratory glassware is only approximate).

See also:
/web-links/

    Supplement

    Гость /28.12.2005 22:55/
    what liquids do not readily dissolve in water subject to a contastant agitiation and are lighter so remain on the surface? (other than oil) preferable also that do not leave a film like oil does on thinngs



    Гость /19.01.2006 21:59/
    can i find the density of liuquids by making and using a straww hydrometer



    n /01.02.2006 04:25/
    Density and solubility are two different things.



    Гость /06.02.2006 07:00/
    ketchup



    Гость /24.03.2006 05:56/
    You should put densities of liquids peole might have heard of or have in their home. For example, honey, Mountain dew, Liquid soap, coffee, or Shampoo.



    Гость /10.06.2008 17:52/
    3MAcetic acid glacial








       
Last modification: 30/07/13

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