Ïîëíàÿ âåðñèÿ ñòðàíèöû  English  

Êàïñàöèí ñèíòàçà îïðåäåëÿåò îñòðîòó ïåðöà

Pages: 1, 2
vladimirchi, 11.09.2006 14:31
Ïåðåö áûë è îñòàåòñÿ êîðîëåì ïðÿíîñòåé. Îñòðîòà, çà êîòîðóþ ñîáñòâåííî è öåíÿò ïëîäû ýòîãî ðàñòåíèÿ, çàâèñèò îò ñîäåðæàíèÿ êàïñàöèíà. Ýòî óíèêàëüíîå âåùåñòâî, âûðàáàòûâàåìîå òîëüêî ïðåäñòàâèòåëÿìè ðîäà Capsicum, ñïîñîáíî íå òîëüêî óëó÷øàòü âêóñ ïèùè. Îíî îáëàäàåò àíòèìèêðîáíûìè, àíòîêñèäàíòíûìè, àíòèàðòðèòíûìè, àíàëüãåòè÷åñêèìè ñâîéñòâàìè, íå ãîâîðÿ óæå î òîì, ÷òî èñïîëüçóåòñÿ â ïîïóëÿðíûõ ñðåäñòâàõ ñàìîçàùèòû. Èññëåäîâàòåëè èç Èíäèè ïðîâåëè äåòàëüíîå èññëåäîâàíèå ñâîéñòâ ôåðìåíòà êàïñèöèí ñèíòàçû (CS). Îíè óñòàíîâèëè, ÷òî ìàêñèìàëüíàÿ àêòèâíîñòü ôåðìåíòà õàðàêòåðíà èìåííî äëÿ ÷àñòåé ïåðöà áîãàòûõ êàïñàöèíîì, îïðåäåëèëè àìèíîêèñëîòíóþ ïîñëåäîâàòåëüíîñòü î÷èùåííîãî ôåðìåíòà, êëîíèðîâàëè ãåí êàïñèöèí ñèíòåòàçû (csy1) è îïðåäåëèëè åãî ïîñëåäîâàòåëüíîñòü. Áûëà òàêæå ïðîäåìîíñòðèðîâàíà ýêñïðåññèÿ csy1 â E. coli. Îäèí èç íàèáîëåå ïðàêòè÷åñêè öåííûõ ðåçóëüòàòîâ ðàáîòû çàêëþ÷àåòñÿ â ÷åòêîì ïîäòâåðæäåíèè òîãî, ÷òî èìåííî ðàçëè÷èÿ ïî àêòèâíîñòè êàïñàöèí ñèíòàçû îïðåäåëÿþò ñïîñîáíîñòü ðàçíûõ ãåíîòèïîâ âûðàáàòûâàòü êàïñàöèí. Ýòà íàõîäêà ñïîñîáíà çíà÷èòåëüíî îáëåã÷èòü ñåëåêöèþ ñîðòîâ ïåðöà ñ «ïðîãðàììèðóåìîé» îñòðîòîé.

Ðåôåðàò ñòàòüè:
http://www.pnas.org/cgi/content/abstract/103/36/13315

êàòåãîðèÿ - 2
GeammaPunny, 27.03.2009 09:28
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