Analytics of Protein-DNA Interactions (Advances in Biochemical Engineering / Biotechnology) 
Advances in Biochemical Engineering/Biotechnology кн.104
Ред. Seitz H.
Springer-Verlag; 2007; 204стр.; ISBN: 3540481478
Organisms are defined by their genetic code – DNA. A detailed knowledge of the mechanisms that duplicate, repair and decode the genetic information is fundamental to our understanding of life itself. Inside the nucleus, DNA is associated with proteins to make chromatin, which forms the template for
function. The processes that control chromatin function are presently mostly described in terms of individual DNA–protein interactions.
In reality, however, such interactions are parts of a complex molecular interaction networks that are highlydynamic in time and space. Understanding such complex biological systems requires the unravelling of these networks and catching them in quantitative and predictive models, based on quantitative experiments.
…
This book focuses on the fascinating possibilities that emerged during the last years to study protein–DNA interactions in vitro. As an essential part of transcriptional activity during development and in tumorgenesis a separate chapter describing the analysis of DNA modification especially methylation of cytosines at their carbon-5 position was included. Besides the established techniques to characterize protein–DNA interactions by surface plasmon resonance, footprinting techniques and electro-mobility shift assays are stillwidely used methods. The organism’s proteome is a dynamic entity containing thousands of elements involved in numerous intricate networking processes that are related to the developmental stages of the life cycle and to virtually every process in the living cell. A number of methods have been developed to study protein binding to nearly all possible target regions within a genome. To give an insight into those techniques a microarray based method is included. Additionally, a chapter describing in detail the potential of mass spectrometry is included.
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