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The enzyme(s) of choice may be engineered into a desired host cell using one or more nucleic acids. For example, a vector may comprise a polynucleotide that encodes a selected molecule as an inactive enzyme that can be activated by cleaving the inactive enzyme at a cleavage site.
The cleavage site may be naturally occurring, but preferably the cleavage site is engineered into the polynucleotide so that it is expressed as a permuted enzyme.
, engineered into.
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