Добрый день. Вопрос такой: можно ли говорить о том, что не зависимо от общей концентрации лиганда в растворе с рецептором связывается только определенный процент молекул лиганда, например 90% от внесенного количества, при условии низкой общей концентрации лиганда, не кооперативном связывании в соотношении 1:1 и линейной зависимости [лиганд] vs регистрируемый сигнал? Этот эффект наблюдали при связывании белков в концентрации до 1 мкг/мл с полимерной поверхностью (Analytical Biochemistry 105, 375-382 (1980). The Adsorptive Characteristics of Proteins for Polystyrene and Their Significance in Solid-Phase lmmunoassays. L. A.CANTARERO, J. E. BUTLER, J.W. OSBORNE), но в данном случае связывание не специфическое. Будет ли этот эффект при специфическом связывании? Спасибо.

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